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An Experimental Study In Combined Effects Of Nd-YAG Laser And Acidulated Phosphate Fluoride On Acid Resistance Of Bovine Tooth Enamel

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Abstract

°á·Ð
ÀúÀÚ´Â ·¹ÀÌÀú Á¶»ç¿Í ºÒ¼ÒµµÆ÷ÀÇ º´¿ëóġ½Ã ¹ý¶ûÁúÀÇ ³»»ê¼º º¯È­¸¦ °üÂûÇϱâ À§ÇØ ¹ß°Å
µÈ ¼ÒÀÇ ÇÏ¾Ç ¿µ±¸ ÀüÄ¡ Áß ¿ì½ÄÀ̳ª °áÇÔÀÌ ¾ø°í °ÇÀüÇÑ 54°³ÀÇ Ä¡¾Æ¸¦ ÀÌ¿ëÇÏ¿© 214°³ÀÇ
½ÃÆíÀ» Á¦ÀÛÇÏ°í ÀÌÁß 70°³¸¦ °í¸¥ µÚ 10°³¸¦ ÇÑ ±ºÀ¸·Î ÇÏ¿© ´ëÁ¶±º ¹× 6°³ÀÇ ½ÇÇ豺À¸·Î
ºÐ·ùÇÏ°í ½ÇÇ豺¿¡´Â ÆÞ½º¹ßÁøÇü Nd-YAG ·¹ÀÌÀú¸¦ ÀÌ¿ëÇØ °¢°¢ 10J/cm2,
20J/cm2, 30J/cm2, 40J/cm2,
50J/cm2 60J/cm2 ¿¡³ÊÁö ¹Ðµµ·Î Á¶»çÇÑ ÈÄ ÁÖ»çÀüÀÚÇö¹Ì°æÀ¸·Î
Ä¡¸éÀ» °üÂûÇÏ°í ¿øÀÚÈí¼öºÐ±¤ÃøÁ¤°è·Î »ê(0.5N HCIO4)¿¡ ¿ëÇØµÈ Ä®½·ÀÌ¿Â
³óµµ¸¦ ÃøÁ¤ÇÏ¿© ¸ÕÀú ³»»ê¼º Áõ°¡¿¡ °¡Àå È¿°úÀûÀÎ ·¹ÀÌÀú ¿¡³ÊÁö ¹Ðµµ¸¦ °áÁ¤ÇÑ ´ÙÀ½, °á
Á¤µÈ ¿¡³ÊÁö ¹ÐµµÀÇ ·¹ÀÌÀú Á¶»ç¿Í ºÒ¼Ò(APF)µµÆ÷ÀÇ º´¿ëóġ ¹æ¹ý¿¡ µû¸¥ ³»»ê¼º º¯È­¸¦
°üÂûÇϱâ À§ÇØ 90°³ÀÇ ½ÃÆíÀ» ºñóġ ´ëÁ¶±º, ºÒ¼Ò¸¸ µµÆ÷ÇÑ ±º, ·¹ÀÌÀú Á¶»ç ÈÄ ºÒ¼ÒµµÆ÷±º
¹× ºÒ¼ÒµµÆ÷ÈÄ ·¹ÀÌÀú Á¶»ç±ºÀ¸·Î ºÐ·ùÇÑ ÈÄ À̵éÀ» °¢°¢ 4ºÐ, 60ºÐ, 24½Ã°£ ºÒ¼ÒµµÆ÷ÇÏ°í
»ê¿¡ ¿ëÇØµÈ Ä®½·ÀÌ¿ÂÀ» °°Àº ¹æ¹ýÀ¸·Î ÃøÁ¤ÇÏ¿© ³»»ê¼ºÀ» ºñ±³ÇÔ°ú µ¿½Ã¿¡ ÁÖ»çÀüÀÚÇö¹Ì°æ
À¸·Î »êó¸®ÇÑ ¹ý¶ûÁúÀÇ Ç¥¸éº¯È­¸¦ °üÂûÇÑ ¹Ù ´ÙÀ½°ú °°Àº °á·ÐÀ» ¾ò¾ú´Ù.
1. ·¹ÀÌÀú Á¶»ç¿¡ ÀÇÇÑ ³»»ê¼º Áõ°¡´Â 40J/cm2 50J/cm2¼­ ºñÁ¶
»ç ´ëÁ¶±º¿¡ ºñÇØ À¯ÀÇÇÏ°Ô Áõ°¡ÇÏ¿´À¸³ª 50J/cm2¿¡¼­ µÎ ±º°£ÀÇ Åë°èÇÐÀûÀÎ
À¯ÀÇÂ÷ ¾øÀÌ °¡Àå Å« ³»»ê¼ºÀ» ³ªÅ¸³»¾ú°í ÁÖ»çÀüÀÚÇö¹Ì°æ»ó 40J/cm2¿¡¼­ ¹ý
¶ûÁúÀÇ ±ÕÀÏÇÑ À¶ÇÕÀ», 50J/cm2¿¡¼­ Ä¡¸éÀÇ ±ÕÀÏÇÑ À¶ÇÕ ¹× ¹Ì¼¼ÇÑ ±Õ¿­À» º¸
¿´´Ù.
2. ¹ý¶ûÁú ¼ÒÁÖ´Â 90ÃÊ µ¿¾È »êºÎ½ÄÈÄ ºñóġ ´ëÁ¶±º, ºÒ¼Ò¸¸ µµÆ÷ÇÑ ±º ¹× ·¹ÀÌÀú
(50J/cm2)¸¸ Á¶»çÇÑ ±º ¸ðµÎ¿¡¼­ ±× ÇüŸ¦ ºÐ¸íÈ÷ º¼ ¼ö ÀÖ¾úÀ¸³ª ·¹ÀÌÀú Á¶
»ç ÈÄ ºÒ¼ÒµµÆ÷ÇÑ ±º¿¡¼­´Â ¹ý¶ûÁú Ç¥¸éÀÌ ºÒ±ÔÄ¢ÇÏ°Ô ºÎ½ÄµÇ¾î ÀϺΠ¹ý¶ûÁúÀº ¿©ÀüÈ÷ ³»»ê
¼ºÀ» ³ªÅ¸³»¾ú´Ù.
3. ºÒ¼ÒµµÆ÷ ½Ã°£ÀÌ Áõ°¡ÇÒ¼ö·Ï ³»»ê¼ºÀº Áõ°¡ÇÏ¿´À¸³ª ·¹ÀÌÀú(50J/cm2) Á¶»ç
ÈÄ 24½Ã°£ ºÒ¼ÒµµÆ÷ÇÑ ±ºÀ» Á¦¿ÜÇÏ¸é °°Àº 󸮹æ¹ýÀ¸·Î 4ºÐ óġÇÑ ±º°ú Åë°èÇÐÀûÀ¸·Î À¯
ÀÇÇÑ Â÷ÀÌ°¡ ¾ø¾ú´Ù.
4. ·¹ÀÌÀú(50J/cm2) Á¶»ç¿Í ºÒ¼ÒµµÆ÷ÀÇ º´¿ëóġ ¹æ¹ý¿¡ µû¸¥ ³»»ê¼º Áõ°¡´Â
·¹ÀÌÀú Á¶»ç ÈÄ ºÒ¼Ò µµÆ÷ÇÑ ±ºÀÌ °¡Àå ¸¹¾ÒÀ¸¸ç ºÒ¼ÒµµÆ÷ ÈÄ ·¹ÀÌÀú Á¶»çÇÑ ±ºÀº À̺¸´Ù
ÆÛ Àû¾î ºÒ¼ÒµµÆ÷³ª ·¹ÀÌÀú Á¶»ç¸¸ ÇÑ ±º°ú ºñ½ÁÇÑ ³»»ê¼ºÀ» º¸¿´´Ù.
#ÃÊ·Ï#
The purpose of this study was to examine the combined effects of a pulsed Nd-YAG
laser and acidulated phosphate fluoride(APF) on acid resistance of bovine tooth enamel.
The materials were 54 freshly extracted bovine permanent teeth with intact smooth
enamel surfaces and sectioned sagitally and horizontally for 214 experimental specimens.
To determine the most effective energy density of laser in increasing acid resistance of
tooth enamel, the author divided 70 specimens into 7 groups including unlased control
and 6 experimental groups. Among them, 60 specimens for 6 experimental groups were
exposed to a pulsed Nd-YAG laser under conditions ranged from 10 to 60J / cm2 with
an increment of 10J/cm2. Total 70 specimens for unlased control and 6
experimental groups were etched with 1ml of 0.5N HCIO4 for 30sec at
room temperature and then the amount of dissolved calcium in the acidic solution was
measured with an atomic absorption spectrophotometer. Scanning electron
microscopic(SEM) observations were also made to examine the enamel surfaces of the
lased teeth with 14 specimens for unlased control and 6 experimental groups. To survey
the combined effects of a pulsed Nd-YAG laser and APF on acid resistance of enamel,
the author divided 90 specimens into 9 groups according to treatment method(APF, only,
APF after lasing, lasing after APF) and fluoride application time(4min, 60min, 24hr).
After determination of the most effective laser-APF combination method on acid
resistance by the amount of dissolved calcium in the acidic solution, the rest 40
specimens for SEM were divided into 20 groups according to treatment
method(untreated control, APF only, the most effective laser + APF method) and etching
time(non-etching, 15, 30, 60, 90 sec). And then, the surfaces of each treated specimens
were also observed with SEM. The results were as follows :
1. The acid resistance of enamel surface increased significantly compared to untreated
control at the laser energy density of 40J/cm2 and 50J/cm2,
but more increased at 50J/cm2 without statistical difference between both
groups. Laser energy density of 40J/cm2 produced the almost uniform
fusion of the lased enamel surface and 50J/cm2 produced the fusion of
enamel surface while that of 50J/cm2 produced the fusion of enamel
surface and fine cracks of a mosaic pattern.
2. In the groups etched for 90 sec, the surface of lasing(50J/cm2) only
group showed its prism structure as in the control, APF only group. But a part of
enamel structure was still resistant to acid in the group treated with APF after leasing.
3. Longer incubation with APF did bring about as increase in acid resistance but there
was no significant increase in acid resistance from 4min treated group with the same
treatment method except the group treated APF for 24hr after
lasing(50J/cm2).
4. The group of APF application after lasing(50J/cm2) caused a remarkable
increase in acid resistance of the enamel, while group of lasing after APF application
showed a lesser effect similar to either APF only or lasing only group.

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